Staining Cells for DNA Content

Staining Cells for DNA Content

Fix cells in Methanol 100%
Keep at -80°C untill staining
Take tubes out and let equilibrate
Spin at 350 x g for 5 minutes and wash with flow buffer
Wash three times 4ml each
Resuspend cells in 100ul of flow buffer with 100ug/ml RNAase
Incubate for 15 minutes at 37°C and add 200 ul of PI for final concentration of 10 ug/ml
Analyze by flow cytometer one day
Note: Make sure that you have same number of cells

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